Recombinant E.coli metC, His-tagged

• Cat.No.: metC-92E
• Product Overview: Recombinant E. coli Cystathionine Beta-Lyase MetC/metC is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Val395) of E. coli metC fused with a 6His tag at the N-terminus.

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Protein Length: 1-395 a.a.
• Description: Cystathionine Beta-Lyase MetC (CBL) belongs to the family of Lyases, specifically the class of carbon-sulfur lyases. CBL catalyzes the chemical reaction converting L-cystathionine to L-homocysteine, NH3 and pyruvate. This reaction depends on pyridoxal phosphate as a cofactor. CBL participates in 5 metabolic pathways: methionine metabolism, cysteine metabolism, selenoamino acid metabolism, nitrogen metabolism, and sulfur metabolism.
• Form: Supplied as a 0.2 μM filtered solution of PBS, pH 7.4
• AA Sequence: MGSSHHHHHHSSGLVPRGSHMADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKHA TRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFIEQGDHVLMTNTA YEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSITMEVHDVPAIVAAVRS VVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDAMIGTAVCNARCWEQLRENAYLM GQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEWLAEHPQVARVNHPALPGSKGHEFWKRDFT GSSGLFSFVLKKKLNNEELANYLDNFSLFSMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTL IRLHIGLEDVDDLIADLDAGFARIV
• Endotoxin: Less than 0.1 ng/µg (1 IEU/µg).
• Purity: Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
• Storage: Store at < -20°C, stable for 6 months after receipt.Please minimize freeze-thaw cycles.