Recombinant E. coli Holliday Junction DNA Helicase RuvB

• Cat.No.: RuvB-2665E
• Product Overview: Recombinant E. coli Holliday Junction DNA Helicase RuvB is expressed inE. coli.

Specification

• Species: E.coli
• Source: E.coli
• Tag: Non
• Description: E. coli RuvB protein forms a complex with RuvA protein at the late stage of homologous recombination and recombination repair and binds specifically to the Holliday structure which is the intermediate of recombination, allowing the migration of Holliday junction using ATP hydrolysis energy and expands the heteroduplex region. RuvB forms a hexamer ring structure and surrounds the double chain DNA and covers RuvA tetramer bound to the Holliday junction from both sides. RuvB is a DNA motor protein which possesses the ATPase activity, activated by DNA and RuvA protein. Its molecular weight is 37 kD and forms a dimer in solution in the physiological condition.
• Form: 50% glycerol, 10 mM Tris-HCl (pH7.5), 2 mM EDTA, 100 mM NaCl, 5 mM mercaptoethanol.
• Purity: RuvB protein over 90% by SDS-PAGE (CBB staining).
• Usage: 1) Studies on homologous recombination mechanism. 2) To make use of the motor protein function that specifically migrates the Holliday junction by forming a complex with RuvA (branch-migration protein).
• Concentration: 1.0 mg/ml (determined by BCA method).
• Storage: -20⁰C.

Gene Information

• Gene Name: ruvB Holliday junction DNA helicase RuvB [ Escherichia coli ED1a ]
• Synonyms:
  ruvB; Holliday junction DNA helicase RuvB
• Gene ID: 7143279
• Protein Refseq: YP_002398010
• UniProt ID: B7MVZ1
• Pathway: Homologous recombination