Recombinant E.coli GrxB, His-tagged

• Cat.No.: grxB-986E
• Product Overview: Recombinant E.coligrxB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques(1-215aa).

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Description: GrxB (Glutaredoxin-2) belongs to the glutaredoxin family. Glutaredoxins are small redox enzymes of approximately one hundred amino-acid residues that use glutathione as a cofactor. Glutaredoxins are oxidized by substrates, and reduced non-enzymatically by glutathione. This protein involved in reducing some disulfides in a coupled system with glutathione reductase. It does not act as hydrogen donor for ribonucleotidereductase.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl
• Molecular Weight: 26.5kDa (235aa) confirmed by MALDI-TOF
• Purity: > 95% by SDS - PAGE
• Concentration: 1mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MKLYIYDHCP YCLKARMIFG LKNIPVELHV LLNDDAETPT RMVGQKQVPI LQKDDSRYMP ESMDIVHYVD KLDGKPLLTG KRSPAIEEWL RKVNGYANKL LLPRFAKSAF DEFSTPAARK YFVDKKEASA GNFADLLAHS DGLIKNISDD LRALDKLIVK PNAVNGELSE DDIQLFPLLR NLTLVAGINW PSRVADYRDN MAKQTQINLL SSMAI
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: grxB glutaredoxin 2 (Grx2) [ Escherichia coli str. K-12 substr. MG1655 ]
• Synonyms: grxB; ECK1049; JW1051; glutaredoxin 2 (Grx2); NP_415582.1; glutaredoxin 2
• Gene ID: 946926
• Protein Refseq: NP_415582