Recombinant E.coli GlsA1, His-tagged

• Cat.No.: glsA1-990E
• Product Overview: Recombinant E.coli glsA1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-310aa).

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Description: GlsA1 (glutaminase1) belongs to the large superfamily of serine-dependent beta-lactamases and penicillin-binding proteins. They are present in most bacteria and eukaryotes and catalyze the hydrolytic deamidation of l-glutamine to l-glutamate and free NH4+.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,10% glycerol, 100mM NaCl
• Molecular Weight: 35.0 kDa (330aa), confirmed by MALDI-TOF
• Purity: > 95% by SDS - PAGE
• Concentration: 1mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MLDANKLQQA VDQAYTQFHS LNGGQNADYI PFLANVPGQL AAVAIVTCDG NVYSAGDSDY RFALESISKV CTLALALEDV GPQAVQDKIG ADPTGLPFNS VIALELHGGK PLSPLVNAGAIATTSLINAE NVEQRWQRIL HIQQQLAGEQ VALSDEVNQS EQTTNFHNRA IAWLLYSAGY LYCDAMEACD VYTRQCSTLL NTIELATLGA TLAAGGVNPL THKRVLQADN VPYILAEMMM EGLYGRSGDW AYRVGLPGKS GVGGGILAVV PGVMGIAAFS PPLDEDGNSV RGQKMVASVA KQLGYNVFKG
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: ybaS glutaminase [ Escherichia coli str. K-12 substr. MG1655 ]
• Synonyms: ybaS; glsA1; ECK0479; JW0474; glutaminase; putative glutaminase; NP_415018.1
• Gene ID: 946187
• Protein Refseq: NP_415018