Recombinant E.coli, G/U mismatch-specific DNA glycosylase; xanthine DNA glycosylase, His-tagged

• Cat.No.: mug-542E
• Product Overview: mug, 1-168aa, E.coli, His tag, E.coli

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• ProteinLength: 1-168 a.a.
• Description: G/U mismatch-specific DNA glycosylase, xanthine DNA glycosylase, also known as mug, belongs to the TDG/mug DNA glycosylase family. It has been proposed that the Mug protein excises 3, N4-ethenocytosine and removes the uracil base from mismatches in the order of U:G>U:A, although the biological role remains unclear. The enzyme Uracil-N-Glycosylase removes uracil from the DNA leaving an AP site. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Recombinant E.coli mug protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol
• Molecular Mass: 21.1 kDa (191aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMVEDILA PGLRVVFCGI NPGLSSAGTG FPFAHPANRF WKVIYQAGFT DRQLKPQEAQ HLLDYRCGVT KLVDRPTVQA NEVSKQELHA GGRKLIEKIE DYQPQALAIL GKQAYEQGFS QRGAQWGKQT LTIGSTQIWV LPNPSGLSRV SLEKLVEAYR ELDQALVVRG R
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: mug?G/U mismatch-specific DNA glycosylase; xanthine DNA glycosylase [?Escherichia coli str. K-12 substr. MG1655?]
• Official Symbol: mug
• Synonyms: mug; dug; ECK3058; JW3040; ygjF; G/U mismatch-specific DNA glycosylase; xanthine DNA glycosylase; G/U mismatch-specific DNA glycosylase; xanthine DNA glycosylase; NP_417540.1; EC 3.2.2.-
• Gene ID: 947560
• Protein Refseq: NP_417540
• Pathway: Base Excision Repair; Base-Excision Repair, AP Site Formation; Depurination