Recombinant E. coli Eco, His-tagged

• Cat.No.: eco-3423E
• Product Overview: Recombinant E. coli Ecotin protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Description: Ecotin (E. coli serine protease inhibitor) inhibits pancreatic serine proteases. This protein inhibits chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a variety of other proteases. The strength of inhibition does not appear to be correlated with particular protease specificity.
• Form: Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 50mM NaCl, 1mM DTT, 10% glycerol.
• Molecular Weight: 18.3 kDa (163aa) confirmed by MALDI-TOF.
• Purity: > 95% by SDS - PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MAESVQPLEK IAPYPQAEKG MKRQVIQLTP QED ESTLKVE LLIGQTLEVD CNLHRLGGKL ENKTLEGWGY DYYVFDKVSS PVS TM MACPD GKKEKKFVTA YLGDAGMLRY NSKLPIVVYT PDNVDVKYRV WK AEE KIDNA VVR.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: eco ecotin, a serine protease inhibitor [ Escherichia coli str. K-12 substr. MG1655 ]
• Synonyms: ecotin, a serine protease inhibitor; ECK2201; eti; JW2197; eco;ecotin, a serine protease inhibitor
• Gene ID: 946700
• Protein Refseq: NP_416713