Recombinant E. coli Dnak Protein

• Cat.No.: DnaK-313E
• Product Overview: DnaK (amino acids 1-638) was amplified by PCR and cloned into an E. coli expression vector. Recombinant E. coli DnaK (1-638) was overexpressed in E. coli and was purified to apparent homogeneity by using conventional column chromatography techniques.

Specification

• Species: E.coli
• Source: E.coli
• Protein Length: 1-638 a.a.
• Description: ATP-regulated binding and release of polypeptide substrates. Hsc56 exhibits specificity toward Hsc62, as Hsc56 does not activate DnaK or Hsc66 ATPase activity.
• Form: Liquid
• Molecular Mass: 69 kDa (638aa)
• AA Sequence: MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQDGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAWVEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDIDDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLLDVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAADNKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLNEDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIESALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDDDVVDAEFEEVKDKK
• Purity: > 85% by SDS-PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 2 to 8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/mL (determined by Bradford assay)
• Storage Buffer: 25mM Tris-HCl buffer (pH 7.5) containing 100mM NaCl, 5mM DTT,10%glycerol

Gene Information

• Gene Name: dnaK chaperone protein DnaK [ Escherichia coli str. K-12 substr. MG1655 ]
• Official Symbol: dnaK
• Synonyms: dnaK; chaperone protein DnaK; ECK0014; groPAB; groPC; groPF; grpC; grpF; seg; chaperone protein DnaK
• Gene ID: 944750
• Protein Refseq: NP_414555
• UniProt ID: P0A6Y8