Recombinant E.coli D-gluconate kinase, His-tagged

• Cat.No.: idnk-610E
• Product Overview: idnk, 1-187aa, E.coli, His tag, E.coli

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Protein Length: 1-187 a.a.
• Description: D-gluconate kinase, thermosensitive, also known as idnk, D-gluconate kinase, thermosensitive, also known as idnk, is a 187 amino acid protein that belongs to the gluconokinase gntK/gntV family and catalyzes the conversion of ATP and D-gluconate to ADP and 6-phospho-D-gluconate. This protein is thought to play a role in gender determination, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X, Y genotype. Recombinant E.coli idnk protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 23.4 kDa (210aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGESFI LMGVSGSGKT LIGSKVAALL SAKFIDGDDL HPAKNIDKMS QGIPLSDEDR LPWLERLNDA SYSLYKKNET GFIVCSSLKK QYRDILRKGS PHVHFLWLDG DYETILARMQ RRAGHFMPVA LLKSQFEALE RPQADEQDIV RIDINHDIAN VTEQCRQAVL AIRQNRICAK EGSASDQRCE
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 1 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: idnK D-gluconate kinase [ Escherichia coli str. K-12 substr. W3110 ]
• Official Symbol: idnk
• Synonyms: idnK; D-gluconate kinase; YP_492406.1; ECK4261:JW4225:b4268
• Gene ID: 12932386
• Protein Refseq: YP_492406
• UniProt ID: AP_004764
• Pathway: Biosynthesis of secondary metabolites; L-idonate degradation; Pentose phosphate pathway