Recombinant E.coli CysH, His-tagged

• Cat.No.: cysH-987E
• Product Overview: Recombinant E.coli cysH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques(1-244aa).

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Description: CysH (Phosphoadenosine phosphosulfate reductase) belongs to the PAPS reductase family, specifically those acting on a sulfur group of donors with a disulfide as acceptor. In enzymology, a cysH is an enzyme that catalyzes the chemical reaction. Three substrates of this enzyme are adenosine 3",5"-bisphosphate, sulfite, and thioredoxin disulfide, whereas its two products are 3"-phosphoadenylyl sulfate and thioredoxin.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl
• Molecular Weight: 30.1kDa (264aa) confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 0.5mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MSKLDLNALN ELPKVDRILA LAETNAELEK LDAEGRVAWA LDNLPGEYVL SSSFGIQAAV SLHLVNQIRP DIPVILTDTG YLFPETYRFI DELTDKLKLN LKVYRATESA AWQEARYGKL WEQGVEGIEK YNDINKVEPM NRALKELNAQ TWFAGLRREQ SGSRANLPVL AIQRGVFKVL PIIDWDNRTI YQYLQKHGLK YHPLWDEGYL SVGDTHTTRK WEPGMAEEET RFFGLKRECG LHEG
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: cysH 3"-phosphoadenosine 5"-phosphosulfate reductase [ Escherichia coli str. K-12 substr. MG1655 ]
• Synonyms: cysH; ECK2757; JW2732; 3"-phosphoadenosine 5"-phosphosulfate reductase; NP_417242.1; EC 1.8.4.8
• Gene ID: 947230
• Protein Refseq: NP_417242