Recombinant E.coli, adenine DNA glycosylase, His-tagged

• Cat.No.: mutY-541E
• Product Overview: mutY, 1-350aa, E.coli, His tag, E.coli

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Protein Length: 1-350 a.a.
• Description: Adenine DNA glycosylase, also known as mutY, is an adenine DNA glycosylase active on DNA substrates containing A/G, A/8-oxoG, or A/C mismatches and also has a weak guanine glycosylase activity on G/8-oxoG-containing DNA. mutY is crucial for the avoidance of mutations resulting from oxidative DNA damage. Increasing levels of mutY in A549 cells exposed to oxygen and infrared radiation leads to improvements in cell survival. It is abundant in neurons where mitochondrial genomes exposed to reactive oxygen species (ROS) that damage DNA must maintain integrity over the entire mammalian life span. Recombinant E.coil mutY protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol
• Molecular Mass: 41.5 kDa (373aa) confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMQASQFS AQVLDWYDKY GRKTLPWQID KTPYKVWLSE VMLQQTQVAT VIPYFERFMA RFPTVTDLAN APLDEVLHLW TGLGYYARAR NLHKAAQQVA TLHGGKFPET FEEVAALPGV GRSTAGAILS LSLGKHFPIL DGNVKRVLAR CYAVSGWPGK KEVENKLWSL SEQVTPAVGV ERFNQAMMDL GAMICTRSKP KCSLCPLQNG CIAAANNSWA LYPGKKPKQT LPERTGYFLL LQHEDEVLLA QRPPSGLWGG LYCFPQFADE ESLRQWLAQR QIAADNLTQL TAFRHTFSHF HLDIVPMWLP VSSFTGCMDE GNALWYNLAQ PPSVGLAAPV ERLLQQLRTG APV
• Purity: >90% by SDS - PAGE
• Storage: Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: mutY?adenine DNA glycosylase [?Escherichia coli str. K-12 substr. MG1655?]
• Official Symbol: mutY
• Synonyms: mutY; ECK2956; JW2928; micA; mutB; adenine DNA glycosylase; adenine DNA glycosylase; NP_417436.1; EC 3.2.2.-; adenine glycosylase; G.C -->T.A transversions
• Gene ID: 947447
• Protein Refseq: NP_417436
• Pathway: Base Excision Repair; Base-Excision Repair, AP Site Formation; Depurination