Recombinant Drosophila Melanogaster ND75 Protein (28-315 aa), His-SUMO-tagged

• Cat.No.: ND75-1806D
• Product Overview: Recombinant Drosophila Melanogaster (Fruit fly) ND75 Protein (28-315 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Protein Description: Partial.

Specification

• Species: Drosophila
• Source: E.coli
• Tag: His&SUMO
• ProteinLength: 28-315 aa
• Description: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 47.8 kDa
• AA Sequence: SAMVAQTPAKAPEKIEVFVDDIPVQVVPGTTVLQAAAQIGVEIPRFCYHERLAVAGNCRMCLVEVEKSPKPVAACAMPVMKGWRIKTNSDLTRKAREGVMEFLLMNHPLDCPICDQGGECDLQDQAMAFGSDRSRFTDINYTGKRAVEDKDIGPLVKTIMTRCIHCTRCVRFASEIAGVDDLGTTGRGNDMQIGTYVEKLFLTELSGNVIDLCPVGALTNKPYSFVARPWEIRKVSSIDVLDAVGSNIVVSTRTNEVLRILPRENEDVNEEWLADKSRFACDGLKRQR
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: ND-75 NADH dehydrogenase (ubiquinone) 75 kDa subunit [ Drosophila melanogaster (fruit fly) ]
• Official Symbol: ND75
• Synonyms: CG2286; Dmel\CG2286; ND75; NDUFS1; NDUS1; NUAM
• Gene ID: 31762
• mRNA Refseq: NM_078528
• Protein Refseq: NP_511083
• UniProt ID: Q94511