Native Bovine Collagen Type II

• Cat.No.: Collagen-55B
• Product Overview: Triplehelical domain of bovine cartilage joint collagen type II. Gnd. HCl extraction, pepsin digestion, saltprecipitation, ion excange chromatography, gelfiltration.

Specification

• Species: Bovine
• Source: Bovine Joint Cartilage
• Tag: Non
• Form: Salt-free, solid lyophilized from 0.5 M acidic acid
• Quality Control Test: SDS and native polyacrylamide gel electrophoresis, collagenase digestion, Immunoblotting with anti-collagen, type I/II/III, rat (Rabbit).
• Storage: Freezer
• Reconstitution: Should be solubilized slowly in 10 mM acidic acid ( 0.2% w/v) and adjusted to neutral pH by adding 2/5 vol. 2.5x Carbonat buffer (0.25 M NaHCO3, 1.25 M NaCl, 0.1% NaN3). Avoid freeze and thaw cycles. Fibril-forming molecules with the molecular formular: α1(II)3.