Recombinant Dog IL6 protein, His-tagged

• Cat.No.: IL6-498D
• Product Overview: Recombinant Dog IL6 protein(Ala18~Met207), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Dog
• Source: HEK293
• Tag: His
• Protein Length: Ala18~Met207
• Tag: N-His
• Form: PBS, pH7.4, containing 5% Trehalose.
• Bio-activity: Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response and also plays a role in fighting infection. It supports the growth of B cells and is antagonistic to regulatory T cells. To test the effect of IL6 on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 8,000 cells/well with 2% serum standard 1640 which contains various concentrations of recombinant dog IL6. After incubated for 3 days, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8(CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 °C.
• Molecular Mass: 33kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: >90%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: IL6 interleukin 6 (interferon, beta 2) [ Canis lupus familiaris ]
• Official Symbol: IL6
• Synonyms: IL6; interleukin 6 (interferon, beta 2); interleukin-6; IL-6
• Gene ID: 403985
• mRNA Refseq: NM_001003301
• Protein Refseq: NP_001003301