Recombinant Dictyostelium Discoideum DNAPKCS Protein (3910-4250 aa), His-Myc-tagged

• Cat.No.: DNAPKCS-2554D
• Product Overview: Recombinant Dictyostelium Discoideum (Slime mold) DNAPKCS Protein (3910-4250 aa) is produced by E. coli expression system. This protein is fused with a 10xHis tag at the N-terminal and a Myc tag at the C-terminal. Protein Description: Partial.

Specification

• Species: Dictyostelium Discoideum
• Source: E.coli
• Tag: His&Myc
• ProteinLength: 3910-4250 aa
• Description: Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Is recruited to DNA ends by the Ku70/Ku80 heterodimer and is involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. This activity is only apparent when DNA damage is administered in G1 phase of the cell cycle. Required for efficient signaling of DNA double-stranded breaks via phosphorylation of H2AX during G1.
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 46.1 kDa
• AA Sequence: ELDYPFLIKGGEDLRLDQRIQQLFGIMNEILKRDTACNKRSLNVTTYQVVPMTSKVGIIEWLNDTKPLREILEEQLAHQLKTPRSNVSISKLESTKYHNDWINSFAKYLKPNSPVGPLYQQMFIHATRDDCAKKLEKQHSKVPENLLQNGIWSLSSSPESYLFIRNSFARSLASFSVCSYVIGIGDRHLENFLISQRDGRLIGIDFGHAFGTATQFLPIPELMPFRLTRQFTSFLRPLDSVGLLNHNMTYTLTALQNQKEILLTTMDVFVKEPLLDWSKLATRLVKEQGKHPKDTKNVWFPKQKIQIAKKKLELVNPAYITLEELSGSVHSGLPYEKALSE
• Purity: > 85% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.

Gene Information

• Synonyms: dnapkcs
• UniProt ID: Q54UC0