Recombinant DENV(type 2,strain New Guinea C/PUO-218 hybrid)(AAC59274.1) E/Envelope Protein, His-tagged
Cat.No. : | Envelope-352D |
Product Overview : | Recombinant Dengue virus (DENV)(type 2,strain New Guinea C/PUO-218 hybrid)(AAC59274.1) (Phe247-Gly675, with two amino acids "MV" at the N-terminal) was expressed with a polyhistidine tag at the C-terminus. |
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Species : | DENV |
Source : | Insect Cells |
Tag : | His |
Protein Length : | Phe247-Gly675 |
Predicted N Terminal : | Met |
Form : | Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol. Normally 5 %- 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Molecular Mass : | The recombinant envelope protein of Dengue virus (DENV)(type 2, strain New Guinea C/PUO-218 hybrid) consists of 442 amino acids and predicts a molecular mass of 49.2 kDa. |
Endotoxin : | < 1.0 EU per μg protein as determined by the LAL method. |
Purity : | > 80 % as determined by SDS-PAGE. |
Storage : | Samples are stable for up to twelve months from date of receipt at-70 centigrade. Store it under sterile conditions at-20 centigrade to-80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Official Symbol | Envelope |
Synonyms | E/Envelope;E Protein |
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Not For Human Consumption!
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