Recombinant Cynomolgus TGFB1 Protein(Leu 30 - Ser 390 (C33S)), His-tagged

• Cat.No.: TGFB1-028C
• Product Overview: Recombinant Cynomolgus TGFB1 Protein(Leu 30 - Ser 390 (C33S))(XP_005589396.1) was expressed in HEK293, with a polyhistidine tag at the N-terminus.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: His
• Protein length: Leu 30 - Ser 390 (C33S)
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Cynomolgus Latent TGF-Beta 1 (C33S), His Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free with a linear range of 1-31 ng/mL.
• Molecular Mass: The protein migrates as 50-60 kDa (precursor proprotein), 38-45 kDa (LAP) and 13 kDa (TGFB1) under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
  >90% as determined by SEC-HPLC.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20°C to -70°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.