Active Recombinant Human TGFB1 Protein

• Cat.No.: TGFB1-157H
• Product Overview: Recombinant Human Transforming Growth Factor beta 1 is produced by our Mammalian expression system and the target gene encoding Ala279-Ser390 is expressed.

Specification

• Description: Transforming Growth Factor β-1 (TGFβ-1) is a secreted protein which belongs to the TGF-β family. TGFβ-1 is abundantly expressed in bone, articular cartilage and chondrocytes and is increased in osteoarthritis (OA). TGFβ-1 performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation and apoptosis. The precursor is cleaved into a latency-associated peptide (LAP) and a mature TGFβ-1 peptide. TGFβ-1 may also form heterodimers with other TGFβ family members. It has been found that TGFβ-1 is frequently upregulated in tumor cells. Mutations in this gene results in Camurati-Engelmann disease.
• Source: CHO Cells
• Species: Human
• Form: Lyophilized from a 0.2 um filtered solution of 50mM Glycine 50mM NaCl pH4.0.
• Bio-activity: Measured by its ability to inhibit the IL-4-dependent proliferation of TF‑1 human erythroleukemic cells. The ED50 for this effect is 4-40 pg/ml.
• AA Sequence: Ala279-Ser390
• Endotoxin: Less than 0.1 ng/ug (1 EU/ug).
• Purity: >95%
• Storage: Lyophilized protein should be stored at < -20 centigrade, though stable at room temperature for 3 weeks.
  Reconstituted protein solution can be stored at 4-7 centigrade for 2-7 days. Aliquots of reconstituted samples are stable at < -20 centigrade for 3 months.
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
• Reconstitution: It is not recommended to reconstitute to a concentration less than 100μg/ml.
  Dissolve the lyophilized protein in distilled water.
  Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Quality Statement: Purity: >95% as determined by reducing SDS-PAGE
• Shipping: The product is shipped at ambient temperature.
  Upon receipt, store it immediately at the temperature listed below.
• Protein length: 279-390 a.a.

Gene Information

• Gene Name: TGFB1 transforming growth factor beta 1 [ Homo sapiens (human) ]
• Official Symbol: TGFB1
• Synonyms: TGF-beta-1; CED; DPD1; TGFB; TGF-b1; TGFB1; CEDLAP; latency-associated peptide; TGFbeta; TGF-beta 1 protein; transforming growth factor beta-1; LAP; IBDIMDE; TGFbeta; TGF-beta1
• Gene ID: 7040
• mRNA Refseq: NM_000660.7
• Protein Refseq: NP_000651.3
• MIM: 190180
• UniProt ID: P04202