Recombinant Cynomolgus/Rhesus IL21R protein(Met1-Pro236), hFc-tagged

• Cat.No.: IL21R-2355C
• Product Overview: Recombinant Cynomolgus / Rhesus IL21R (XP_005591578.1) (Met1-Pro236) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus. Cynomolgus and Rhesus IL21R sequences are identical.

Specification

• Source: HEK293
• Species: Cynomolgus/Rhesus
• Tag: Fc
• Protein length: Met1-Pro236
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized human IL21 at 10 μg/mL (100 μL/well) can bind Cynomolgus IL21R-Fc. The EC50 of Cynomolgus IL21R-Fc is 0.11-0.25 μg/mL.
• Molecular Mass: The recombinant cynomolgus / rhesus IL21R consists 458 amino acids and predicts a molecular mass of 52 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.