Recombinant Cynomolgus REG1A protein(Met1-Asn166), His-tagged

• Cat.No.: REG1A-89C
• Product Overview: Recombinant Cynomolgus REG1A (G8F4F8) (Met1-Asn166) was expressed in HEK293 with a polyhistidine tag at the C-terminus.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: His
• ProteinLength: Met1-Asn166
• Form: Lyophilized from sterile PBS, PH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant cynomolgus REG1A comprises 155 amino acids and has a calculated molecular mass of 177 KDa. The apparent molecular mass of it is approximately 19 and 21 KDa respectively in SDS-PAGE.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Case Study

Case 1: Mao H, et al. Int Immunopharmacol. 2021

In various gastrointestinal inflammatory conditions, REG1A protein levels were found to be elevated, but its specific role and regulatory mechanisms in inflammatory bowel disease (IBD) are not yet fully understood. This research revealed a significant increase in REG1A in the inflamed colorectal tissues of IBD patients. This overexpression of REG1A in intestinal epithelial cells (IECs) was shown to reduce inflammation, enhance cell proliferation, and prevent epithelial cell death. The activation of the JAK/STAT3 signaling pathway by IL-6 and IL-22 was identified as a key mechanism for REG1A transcription. Furthermore, systemic administration of REG1A lentivirus in mice significantly reduced DSS-induced intestinal inflammation and preserved the integrity of the intestinal mucosal barrier.

Fig1. Western blot analysis of REG1A protein level in paired inflamed and healthy control colonic tissues.

Fig2. RT-PCR was performed to determine the mRNA level of REG1A in HT-29 cells stimulated with various cytokines and growth factors.

Case 2: Zhou M, et al. Cell Death Dis. 2023

Aerobic glycolysis is a characteristic feature of colorectal cancer (CRC), but the regulatory factors controlling glycolysis in CRC are not yet fully understood. This study discovered that levels of Regenerating islet-derived protein 1-alpha (REG1α) were markedly higher in CRC tissues and serum, correlating with lymph node metastasis, advanced tumor stages, and poor prognosis in CRC patients. The overexpression of REG1α enhanced tumorigenic characteristics such as cell proliferation, cell cycle progression, migration, invasion, and glycolysis, while its absence reduced these malignant traits and glucose metabolism in CRC cells. REG1α was found to stimulate CRC proliferation and metastasis by upregulating glycolysis through the β-catenin/MYC axis. Additionally, the malignant effects of REG1α could be neutralized by inhibiting the Wnt/β-catenin/MYC axis or glycolysis with specific inhibitors. Furthermore, REG1α expression was regulated by METTL3 in an m6A-dependent manner.

Fig1. Validation of the interaction between REG1α and β-catenin in CRC cells by co-immunoprecipitation.

Fig2. qRT-PCR detection of REG1α upon inhibition wild type in HCT116 and SW620 cells.

Application

Recombinant Cynomolgus REG1A protein is a valuable tool in biomedical research, with applications spanning from cell biology to regenerative medicine. It plays a crucial role in cell proliferation, differentiation, and tissue regeneration, making it instrumental in studying various biological processes and diseases. This protein can be used to promote tissue repair and regeneration, especially in liver, pancreas, and gastrointestinal tissues, and as a target for drug development to treat related diseases. Additionally, it has potential applications in immunology, cancer research, gene therapy, and as a biomarker for disease diagnosis and prognosis.

Furthermore, the recombinant Cynomolgus REG1A protein is also significant in toxicology studies, assessing the impact of chemicals and environmental factors on cells and tissues, and in stem cell research, where it can be used to study the self-renewal and differentiation of stem cells. Its broad potential in biomedicine highlights the importance of further research to validate its specific applications and effects.

Fig1. Involvement of REG family proteins in inflammation-associated carcinogenesis. (Chao Sun, 2021)