Recombinant Cynomolgus PDCD1 protein, hFc-tagged

• Cat.No.: PDCD1-5743C
• Product Overview: Recombinant Cynomolgus PDCD1 protein(B0LAJ3)(Pro21-Gln167), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: C-hFc
• Protein length: Pro21-Gln167
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Cynomolgus PD-1, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-PD1 Antibody, hFc Tag with the EC50 of 25.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 42.4 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.