Recombinant Cynomolgus Monkey Fas Protein, Fc tagged

• Cat.No.: FAS-513C
• Product Overview: Recombinant Cynomolgus Monkey Fas/TNFRSF6/CD95 (Gln26-Asp173) with Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Chinese Hamster Ovary cell line.

Specification

• Species: Cynomolgus
• Source: CHO
• Tag: Fc
• Protein Length: 26-173 aa
• Description: Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase CASP8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs CASP8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both (By similarity).
• Tag: Fc
• Form: Disulfide-linked homodimer
• Bio-activity: Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 40.0-240 ng/mL.
• Molecular Mass: 43 kDa
• N-terminal Sequence Analysis: Gln26, inferred from deblocking revealing Val27
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Shipping: The product is shipped with polar packs.
• Reference: 1. Bouillet, P. and L.A. O''Reilly (2009) Nat. Rev. Immunol. 9:514.
  2. Strasser, A. et al. (2009) Immunity 30:180.
  3. Ashkenazi, A. and V. Dixit (1999) Curr. Opin. Cell Biol. 11:255.

Gene Information

• Gene Name: FAS Fas cell surface death receptor [ Macaca fascicularis (crab-eating macaque) ]
• Official Symbol: FAS
• Synonyms: FAS; Fas cell surface death receptor; APT1; TNFRSF6; tumor necrosis factor receptor superfamily member 6; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor; Tumor necrosis factor receptor superfamily member 6; death receptor Fas (APO-1/CD95)
• Gene ID: 102140989
• UniProt ID: Q9TSN4