Recombinant Cynomolgus MME protein, His-tagged

• Cat.No.: MME-1627C
• Product Overview: Recombinant Cynomolgus MME protein(G7NZK8)(Asp53-Trp750), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: N-His
• Protein Length: Asp53-Trp750
• Tag: N-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPPGFSAFK (Dnp) -OH. The specific activity is > 1200 pmoles/min/μg.
• Molecular Mass: The protein has a predicted MW of 80.69 kDa. Due to glycosylation, the protein migrates to 81-110 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.