Recombinant Cynomolgus IL18R1 protein, His-tagged

• Cat.No.: IL18R1-1466C
• Product Overview: Recombinant Cynomolgus IL18R1 protein(A0A2K5V3J9)(Cys22-Gly329), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: C-His
• Protein Length: Cys22-Gly329
• Tag: C-His
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Cynomolgus IL-18 R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 2.02 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 36.45 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC.
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.