Recombinant Cynomolgus IL18, His tagged

• Cat.No.: IL18-385C
• Product Overview: Recombinant Cynomolgus IL18 (Ala 2-Asp 193), fused with an N-terminal polyhistidine tag, was produced in E. coli.

Specification

• Species: Cynomolgus
• Source: E.coli
• Tag: His
• Protein Length: 2-193 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4
• Molecular Mass: The recombinant cynomolgus IL18 consists of 203 amino acids and has a calculated molecular mass of 23.7 kDa. The apparent molecular mass of the recombinant protein is approximately 24 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Stability: Samples are stable for up to twelve months from date of receipt at -70oC.
• Storage: Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: IL18 interleukin 18 (interferon-gamma-inducing factor) [ Macaca fascicularis (crab-eating macaque) ]
• Official Symbol: IL18
• Gene ID: 102135101
• mRNA Refseq: XM_005579635
• Protein Refseq: XP_005579692

Q&As

What are the difference between IL18-01HG, IL18-153HG and IL18-500H. (sequence, biological activity, QC, or what do you propose to buy)

IL18-01HG: Sequence: a.a.37-193 Endotoxin: <20 EU/mg Bio-activity: >5.0 x 10^4 IU/mg QC: Activity test, SDS-PAGE, LAL, SEC-HPLC IL18-153HG: Protein length: Tyr37-Asp193 AA Sequence: YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIISMYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDIIFFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIMFTVQNEDVD Bio-activity: 1.0*10^6IU Measured by its ability to induce IFN-gamma secretion by KG‐1 human acute myelogenous leukemia cells in the presence of TNF-alpha. QC: Activity test, SDS-PAGE, LAL IL18-500H Recombinant Human IL18 will be custom produced upon order. All the sequence, purity, activity, and QC procedures will be optional and decided by our customer.