Recombinant Human IL18, GST tagged
Cat.No. : | IL18-574H |
Product Overview : | Recombinant Human IL18 (Q14116) (Met1-Asp193), fused with the GST tag at the N-terminus, was produced in E. coli. |
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Species : | Human |
Source : | E.coli |
Tag : | His |
Protein Length : | 1-193 a.a. |
Predicted N Terminal : | Met |
Form : | Lyophilized from sterile PBS, pH 7.5Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. |
Molecular Mass : | The recombinant human IL18/GST chimera consists of 419 amino acids and has a predicted molecular mass of 48.6 kDa. It migrates as an approxiamtely 45 kDa band in SDS-PAGE under reducing conditions. |
Endotoxin : | < 1.0 eu per μg of the protein as determined by the LAL method. |
Purity : | >85 % as determined by SDS-PAGE |
Stability : | Samples are stable for up to twelve months from date of receipt at -70oC. |
Storage : | Store it under sterile conditions at -20oC~-70oC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution. Centrifuge the vial at 4℃ before opening to recover the entire contents. |
Gene Name | IL18 interleukin 18 (interferon-gamma-inducing factor) [ Homo sapiens ] |
Official Symbol | IL18 |
Gene ID | 3606 |
mRNA Refseq | NM_001243211 |
Protein Refseq | NP_001230140 |
MIM | 600953 |
UniProt ID | Q14116 |
Chromosome Location | 11q22.2-q22.3 |
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Not For Human Consumption!
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Q&As (1)
Ask a questionIL18-01HG: Sequence: a.a.37-193 Endotoxin: <20 EU/mg Bio-activity: >5.0 x 10^4 IU/mg QC: Activity test, SDS-PAGE, LAL, SEC-HPLC IL18-153HG: Protein length: Tyr37-Asp193 AA Sequence: YFGKLESKLS VIRNLNDQVL FIDQGNRPLF EDMTDSDCRD NAPRTIFIISMYKDSQPRGM AVTISVKCEK ISTLSCENKI ISFKEMNPPD NIKDTKSDIIFFQRSVPGHD NKMQFESSSY EGYFLACEKE RDLFKLILKK EDELGDRSIMFTVQNEDVD Bio-activity: 1.0*10^6IU Measured by its ability to induce IFN-gamma secretion by KG‐1 human acute myelogenous leukemia cells in the presence of TNF-alpha. QC: Activity test, SDS-PAGE, LAL IL18-500H Recombinant Human IL18 will be custom produced upon order. All the sequence, purity, activity, and QC procedures will be optional and decided by our customer.
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