Recombinant Cynomolgus HLA-G protein, His-Avi-tagged

• Cat.No.: HLA-G-503C
• Product Overview: Recombinant Cynomolgus HLA-G Protein(E0WKX9(HLA-G)&Q8SPW0(B2M)&RIIPRHLQL)(Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide) is expressed from Expi293 with His tag and Avi tag at the C-terminal.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Avi&His
• Protein Length: Gly25-Thr305(HLA-G),Ile21-Met119(B2M) and RIIPRHLQL peptide
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Molecular Mass: The protein has a predicted MW of 50.6 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -20 to -80°C for 3-6 months in unopened state after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please avoid freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.