Recombinant Cynomolgus GDF15 protein, hFc-tagged, Biotinylated, Primary Amine Labeling

• Cat.No.: GDF15-5644C
• Product Overview: Primary Amine Labeling Biotinylated Recombinant Cynomolgus GDF15 protein(G7PWZ3)(Ala197-Val308), fused with N-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Cynomolgus
• Source: HEK293
• Tag: Fc
• Protein Length: Ala197-Val308
• Tag: N-hFc
• Conjugation/Label: Biotin; Primary Amine Labeling
• Form: Supplied as 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Cynomolgus GFRAL, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Cynomolgus GDF15, hFc Tag with the EC50 of 11.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 37.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.