Recombinant CRM197

• Cat.No.: CRM197-2780C
• Product Overview: Recombinant CRM197is expressed in Pseudomonas fluorescens. MW = 58.4 kDa.

Specification

• Source: Pseudomonas Fluorescens
• Tag: Non
• Description: CRM197 is a non-toxic mutant of diphtheria toxin1,2 having a single amino acid substitution of glutamic acid for glycine. CRM197 is a well defined protein and functions as a carrier for polysaccharides and haptens making them immunogenic. It is utilized as a carrier protein in a number of approved conjugate vaccines for diseases such as meningitis and pneumococcal bacterial infections.
• Endotoxin: < 100 EU/mg of protein by LAL method.
• Purity: > 95% CRM197 by SDS-PAGE. A and B chain content < 5% of total CRM197.
• Dimer: < 5%.
• Storage and use: CRM197 is lyophilized from a 0.2 μm-filtered solution containing 4 mg/mL CRM197, 50 mg/mL sucrose, 0.22 mg/mL sodium phosphate monobasic, 3 mg/mL sodium phosphate dibasic, 0.055 mg/mL polysorbate 80. Store at 2-8°C upon receipt. Before use, aseptically reconstitute lyophilized CRM197 with sterile deionized water to a concentration of 4 mg/mL CRM197. Reconstituted CRM197 should be stored at 2-8°C.

Gene Information

• Gene Name: tox diphtheria toxin precursor [ Corynebacterium diphtheriae NCTC 13129 ]
• Synonyms: Tox; diphtheria toxin precursor; EC 2.4.2.36
• Gene ID: 2650491
• Protein Refseq: NP_938615