Recombinant COVID-19 Spike S1 (D614G) protein, His-Avi-tagged

• Cat.No.: Spike-5755V
• Product Overview: Recombinant COVID-19 Spike S1 (D614G) protein(QHD43416.1)(Gln14-Arg683(D614G)), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: COVID-19
• Tag: C-His-Avi
• Protein length: Gln14-Arg683(D614G)
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized SARS-CoV-2 S1 (D614G) , His Tag at 2μg/ml (100μl/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: S spike protein [ Middle East respiratory syndrome-related coronavirus ]
• Official Symbol: S
• Synonyms: Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1
• Gene ID: 14254594
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2