Recombinant COVID-19 Spike RBD (Omicron B.1.1.529) protein, His-tagged

• Cat.No.: Spike-2744V
• Product Overview: Recombinant COVID-19 Spike RBD (Omicron B.1.1.529) protein(QHO60594.1)(Arg319-Phe541(G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H)), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: COVID-19
• Tag: C-His
• Protein length: Arg319-Phe541(G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H)
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized SARS-COV-2 Spike RBD (Omicron B.1.1.529) , His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 9.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 26.2 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per ug by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: S spike protein [ Middle East respiratory syndrome-related coronavirus ]
• Official Symbol: S
• Synonyms: Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1
• Gene ID: 14254594
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2