Recombinant COVID-19 Spike S1 (W152C, L452R, D614G) protein(Met1-Arg685), His-tagged

• Cat.No.: Spike-1282V
• Product Overview: Recombinant COVID-19 Spike S1 (YP_009724390.1, with mutations W152C, L452R, D614G) (Met1-Arg685) was expressed in HEK293 with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as CAL.20C or B.1.429 lineage) which emerged in United States.

Specification

• Source: HEK293
• Species: COVID-19
• Tag: His
• Protein length: Met1-Arg685
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Immobilized human ACE2 protein (mFc tag) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S1 (W152C, L452R, D614G) Protein (His Tag), the EC50 of SARS-CoV-2 Spike S1 (W152C, L452R, D614G) Protein (His Tag) is 35-200 ng/mL.
• Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Spike S1 consists of 681 amino acids and predicts a molecular mass of 76.4 kDa. As a result of glycosylation, it migrates as an approximately 93.5 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ]
• Official Symbol: Spike
• Synonyms: Spike; S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1
• Gene ID: 43740568
• Protein Refseq: YP_009724390.1
• UniProt ID: P0DTC2