Active Recombinant COVID-19 Plpro protein, His-tagged

• Cat.No.: Plpro-198V
• Product Overview: Recombinant COVID-19 Plpro Protein (Glu1564-Val1880), fused to His-tag at the N-terminus, was expressed in E.coli.

Specification

• Species: COVID-19
• Source: E.coli
• Tag: His
• Protein Length: Glu1564-Val1880
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to cleave a fluorogenic peptide substrate, Arg-Leu-Arg-Gly-Gly-AMC (RLRGGAMC).The Specific Activity is >80pmols/min/μg
• Molecular Mass: The recombinant SARS-CoV-2 (2019-nCoV) Plpro / papain-like protease (His Tag) consists of 324 amino acids and predicts a molecular mass of 36.79 kDa.
• Purity: > 90 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.