Recombinant COVID-19 NSP1 protein, His-tagged

• Cat.No.: NSP1-002V
• Product Overview: Recombinant COVID-19 NSP1 protein (Met1-Gly180) was fused to his-tag at the C-terminus and expressed in E.coli.

Specification

• Species: Sars-CoV-2
• Source: E.coli
• Tag: His
• Protein Length: Met1-Gly180
• Description: The viral nonstructural protein 1 (nsP1) is the only membrane-associated protein that anchors the replication complex to the cellular membranes. NSP1 inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.
• Predicted N Terminal: Met
• Form: Lyophilized from 0.22 μm filtered solution in PBS, Arginine, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 21.7 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE).
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Shipping: Powder,RT

Gene Information

• Gene Name: orf1ab
• Official Symbol: orf1ab
• Synonyms: pp1ab
• Gene ID: 1489680
• Protein Refseq: NP_828860.2
• UniProt ID: P0C6X7