Recombinant COVID-19 3CL-Mpro Protein, His-tagged

• Cat.No.: 3CL-Mpro-135V
• Product Overview: Recombinant COVID-19 3CL-Mpro Protein(YP_009725295.1), fused with C-terminal His tag, was expressed in E. coli.

Specification

• Species: COVID-19
• Source: E.coli
• Tag: His
• Form: Liquid in sterile 50 mM Tris, 50 mmol NaCl, 50% Glycerol, pH 8.0.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Bio-activity: Measured by its ability to cleave a peptide substrate, Dabcyl-KTSAVLQSGFRKME-Edans. Specific activity of 29.5 pmol/min/μg.
• Molecular Mass: It migrates as approximately 33.7 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: ≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC.
• Concentration: 0.51 mg/mL