Recombinant CMV UL55

• Cat.No.: UL55-179C
• Product Overview: A DNA sequence encoding the extracellular domain (Met 1-Lys 700) linked with the cytoplasmic domain (Arg 777-Val 907) of human CMV gB (AAA45920.1) was expressed, fused with five additional amino acids (DDDDK) at the C-terminus.

Specification

• Species: CMV
• Source: Human Cells
• Tag: Non
• Protein Length: Met1-Lys700 & Arg777-Val907
• Description: Human cytomegalovirus(CMV) (human herpesvirus 5) glycoprotein B is a 907-amino acid glycoprotein encoded by the ORF of UL55. It is the most abundant component of the envelope with at least two defined neutralizing epitopes, a target of neutralizing antibodies and an essential replication component. The synthesized gB precursor matures into a 130-160 kDa glycoprotein by acquiring N-linked glycosylation modifications, and is cleaved by the cellular protease furin into two components, a 93-116 kDa N-terminal fragment and a 55 kDa C-terminal fragment linked by a disulfide-bond, which is presented on the viral envelope as well as on the surface of virus-infected cells as a covalently associated homodimer. gB plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. It triggers penetration of cells and enhances the spread of infection in nonpolarized human fibroblasts. Theinteraction of gB with the non-heparin receptor CD209/DC-SIGN is the initiation of intracellular signaling and activation of the IFN-responsive pathway. In addition, gB is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium.
• Form: Lyophilized from sterile PBS, pH7.4.
• Molecular Mass: The recombinant glycoprotein B of human CMV consists of 813 amino acids and has a predicted molecular mass of 92 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of gB is approximately 130-140 kDa due to glycosylation.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: Hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: UL55 type 1 membrane protein; possible membrane fusogen; binds cell surface heparan sulphate; involved in cell entry; involved in cell-to-cell spread [ Human herpesvirus 5 (Human cytomegalovirus) ]
• Official Symbol: UL55
• Synonyms: UL55; type 1 membrane protein; possible membrane fusogen; binds cell surface heparan sulphate; involved in cell entry; involved in cell-to-cell spread; envelope glycoprotein B
• Gene ID: 3077424
• Protein Refseq: YP_081514
• UniProt ID: Q6SW76