Recombinant Chicken IL10 Protein, His&GST-tagged

• Cat.No.: IL10-999C
• Product Overview: Recombinant Chicken IL10 Protein (Leu22-Lys175) with His&GST tag was expressed in E. coli.

Specification

• Species: Chicken
• Source: E.coli
• Tag: GST&His
• Protein Length: Leu22-Lys175
• Description: The protein encoded by this gene is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. Mutations in this gene are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 48.31 kDa; Accurate Molecular Mass: 48 kDa
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: IL10 interleukin 10 [ Gallus gallus (chicken) ]
• Official Symbol: IL10
• Synonyms: IL10; interleukin 10; IL-10; interleukin-10
• Gene ID: 428264
• mRNA Refseq: NM_001004414
• Protein Refseq: NP_001004414
• UniProt ID: Q6A2H4