Recombinant Chicken GAPDH protein, MBP&His-Avi-tagged, Biotinylated

• Cat.No.: GAPDH-5453C
• Product Overview: Biotinylated Recombinant Chicken GAPDH protein(P00356)(2-333aa), fused with N-terminal MBP tag and C-terminal His and Avi tag, was expressed in E.coli.

Specification

• Source: E.coli
• Species: Chicken
• Tag: N-MBP&C-His-Avi
• Protein length: 2-333aa
• Conjugation/Label: Biotin
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 83.3 kDa
• AASequence: VKVGVNGFGRIGRLVTRAAVLSGKV QVVAINDPFIDLNYMVYMFKYDSTH GHFKGTVKAENGKLVINGHAITIFQ ERDPSNIKWADAGAEYVVESTGVFT TMEKAGAHLKGGAKRVIISAPSADA PMFVMGVNHEKYDKSLKIVSNASCT TNCLAPLAKVIHDNFGIVEGLMTTV HAITATQKTVDGPSGKLWRDGRGAA QNIIPASTGAAKAVGKVIPELNGKL TGMAFRVPTPNVSVVDLTCRLEKPA KYDDIKRVVKAAADGPLKGILGYTE DQVVSCDFNGDSHSSTFDAGAGIAL NDHFVKLVSWYDNEFGYSNRVVDLM VHMASKE
• Purity: Greater than 90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C.

Reviews

07/14/2022

Customer service was patient, prompt and accurate in answering my queries.

11/26/2021

High protein concentration, good quality, worth buying!

01/02/2021

The product brochure is very detailed and has comprehensive basic information about the GAPDH protein.

Q&As

Is GAPDH expression stable across tissues?

GAPDH is highly expressed in all tissues with relatively stable expression and is often used as an internal reference protein.

What diseases has GAPDH been closely associated with the study of?

GAPDH is closely related to the study of hepatocellular carcinoma and can be used as one of the indicators for the diagnosis of hepatocellular carcinoma.

What is the relationship between GAPDH and HDAC1?

GAPDH binds to HDAC1.

What are the biological functions of GAPDH?

GAPDH binds to matrix proteins, regulates host immune responses, and plays a role in pathogen virulence and surface localization, among others.

What are the effects of affecting protein expression of GAPDH?

Interference with GAPDH protein expression results in telomere shortening.