Recombinant Cattle VEGFA protein, His & T7-tagged

• Cat.No.: VEGFA-590C
• Product Overview: Recombinant Cattle VEGFA aa. (Ala27~Arg145 (Accession # P15691)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Cattle
• Source: E.coli
• Tag: His&T7
• Protein Length: Ala27~Arg145
• Description: This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulfide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells, and is essential for both physiological and pathological angiogenesis. It is upregulated in many known tumors and its expression is correlated with tumor stage and progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study showed that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is antiangiogenic.
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 17.8kDa
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 7.6.
• Applications: SDS-PAGE; WB; ELISA; IP
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: VEGFA vascular endothelial growth factor A [ Bos taurus (cattle) ]
• Official Symbol: VEGFA
• Synonyms: VEGFA; vascular endothelial growth factor A; VPF; VEGF; VEGF-A; eVEGF120; eVEGF164; vascular endothelial growth factor A; vascular endothelial growth factor 164; vascular permeability factor
• Gene ID: 281572
• mRNA Refseq: NM_001316955.1
• Protein Refseq: NP_001303884.1
• UniProt ID: P15691