Recombinant Cattle SERPINF1 Protein, His-tagged

• Cat.No.: SERPINF1-2686C
• Product Overview: Recombinant Cattle SERPINF1 Protein (Ser33-Thr416) with a N-His tag was expressed in E. coli.

Specification

• Description: Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.
• Source: E. coli
• Species: Cattle
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 46.6 kDa
  Accurate Molecular Mass: 44 kDa
• Protein length: Ser33-Thr416
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: SERPINF1 serpin family F member 1 [ Bos taurus (cattle) ]
• Official Symbol: SERPINF1
• Synonyms: SERPINF1; serpin family F member 1; PEDF; pigment epithelium-derived factor
  serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1
  serpin F1
• Gene ID: 281386
• mRNA Refseq: NM_174140
• Protein Refseq: NP_776565
• UniProt ID: Q95121