Active Recombinant Full Length Human SERPINF1, His-tagged

• Cat.No.: SERPINF1-378H
• Product Overview: Recombinant Human SERPINF1 (Accession # AAK92491) Met1-Pro418, fused with a C-terminal 6-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Human
• Tag: His
• Predicted N Terminal: Gln20 is predicted: no results obtained, sequencing might be blocked
• Form: Lyophilized from a 0.2 µm filtered solution in Tris and NaCl.
• Bio-activity: Measured by its ability to enhance the adhesion of Saos-2 human osteosarcoma cells to bovine Collagen I coated plate. Eth, E.K. et al. (2007) Cancer Gene Therapy. 14:616.The ED50 for this effect is typically 0.15-0.75 µg/mL.
• Molecular Mass: Recombinant Human SERPINF1, His-tagged has a calculated MW of 45 kDa. In SDS-PAGE migrates as 45-53 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Protein length: 1-418 a.a.

Gene Information

• Gene Name: SERPINF1 serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1 [ Homo sapiens ]
• Official Symbol: SERPINF1
• Synonyms: SERPINF1; serpin peptidase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; PEDF, serine (or cysteine) proteinase inhibitor, clade F (alpha 2 antiplasmin, pigment epithelium derived factor), member 1; pigment epithelium-derived factor; EPC 1; PIG35; pigment epithelium derived factor; proliferation inducing protein 35; serpin F1; cell proliferation-inducing gene 35 protein; serine (or cysteine) proteinase inhibitor, clade F (alpha-2 antiplasmin, pigment epithelium derived factor), member 1; OI6; OI12; PEDF; EPC-1
• Gene ID: 5176
• mRNA Refseq: NM_002615
• Protein Refseq: NP_002606
• MIM: 172860
• UniProt ID: P36955
• Chromosome Location: 17p13.3