Recombinant Cattle S100A12 protein, His-tagged

• Cat.No.: S100A12-1478C
• Product Overview: Recombinant Cattle S100A12 aa. (Thr2~Glu92) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Source: E. coli
• Species: Cattle
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 14kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Thr2~Glu92
• Endotoxin: <1.0EU per 1µg (determined by the LAL method).
• Purity: >98%
• Characteristic: The isoelectric point is 5.9.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive
  Labeling.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200µg/mL
• Storage buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.

Gene Information

• Gene Name: S100A12 S100 calcium binding protein A12 [ Bos taurus (cattle) ]
• Official Symbol: S100A12
• Synonyms: CAGC; CAAF1; CO-AG; EN-RAGE; RAGE-binding protein; calcium-binding protein in amniotic fluid 1; calgranulin-C; cornea-associated antigen; extracellular newly identified RAGE-binding protein; protein S100-A12
• Gene ID: 282467
• mRNA Refseq: NM_174651.3
• Protein Refseq: NP_777076.1
• UniProt ID: P79105