Recombinant Cattle FGF2 Protein, His-tagged

• Cat.No.: FGF2-2185C
• Product Overview: Recombinant Cattle FGF2 Protein (Pro10-Ser155) with a N-His tag was expressed in E. coli.

Specification

• Description: The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF.
• Source: E. coli
• Species: Cattle
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 20.1 kDa
  Accurate Molecular Mass: 20/23 kDa
• Protein length: Pro10-Ser155
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 97%
• Applications: Cell culture; Activity Assays.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: FGF2 fibroblast growth factor 2 [ Bos taurus (cattle) ]
• Official Symbol: FGF2
• Synonyms: FGF2; fibroblast growth factor 2; BFGF; FGF-2; HBGF-2; fibroblast growth factor 2
  basic fibroblast growth factor
  fibroblast growth factor 2 (basic)
  heparin-binding growth factor 2
• Gene ID: 281161
• mRNA Refseq: NM_174056
• Protein Refseq: NP_776481
• UniProt ID: F1N3T9