Active Recombinant Mouse Fgf2 Protein

• Cat.No.: Fgf2-060M
• Product Overview: Purified recombinant protein of Mouse fibroblast growth factor 2 (Fgf2) (Ala11-Ser154) without tag was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Protein Length: 11-154 a.a.
• Description: Broad expression in mammary gland adult (RPKM 4.1), ovary adult (RPKM 3.7) and 18 other tissues.
• Bio-activity: The ED50 is 0.3 to 2 ng/ml, corresponding to a specific activity of 3.3 to 0.5 x 10^6 units/mg, determined by the dose dependent stimulation of 3T3 cell proliferation.
• Molecular Mass: 16.2 kDa
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Purity: >98%, as determined by Coomassie stained SDS-PAGE.
• Stability: Unopened vial can be stored between 2 centigrade and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 centigrade and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: Protein formulated and vialed at >50 ug/mL in specified buffer
• Storage Buffer: 10mM NaH2PO4, 150mM NaCl, pH7.2, 1mM DTT, 0.5mM EDTA

Gene Information

• Gene Name: Fgf2 fibroblast growth factor 2 [ Mus musculus (house mouse) ]
• Official Symbol: Fgf2
• Synonyms: Fgf2; fibroblast growth factor 2; Fgfb; bFGF; Fgf-2; fibroblast growth factor 2; HBGF-2; basic fibroblast growth factor; heparin-binding growth factor 2
• Gene ID: 14173
• mRNA Refseq: NM_008006
• Protein Refseq: NP_032032
• UniProt ID: P15655