Recombinant Canine HGF protein(Met1-Ser730)

• Cat.No.: HGF-1045C
• Product Overview: Recombinant canine HGF (NP_001002964.1) (Met1-Ser730) was expressed in HEK293.

Specification

• Species: Canine
• Source: HEK293
• Tag: Non
• Protein Length: 1-730 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation. The ED50 for this effect is typically 1-4 ng/mL.
• Molecular Mass: The recombinant canine HGF consists 699 amino acids and predicts a molecular mass of 79.9 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.