Recombinant Canine CD274 protein, hFc-tagged

• Cat.No.: CD274-1058C
• Product Overview: Recombinant Canine CD274 protein(NP_001278901.1)(Met1-Arg236), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Canine
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Arg236
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Molecular Mass: The recombinant canine CD274 consists of 456 amino acids and predicts a molecular mass of 51.5 kDa. As a result of glycosylation, it migrates as an approximately 77.93 and 149.69 kDa band in SDS-PAGE under reducing conditions, corresponding to the monomer and homodimer.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.