Recombinant Canine CD274 protein, His-tagged

• Cat.No.: CD274-0620C
• Product Overview: Recombinant Canine CD274 protein(Phe19-Arg236), fused with His tag, was expressed in HEK293.

Specification

• Species: Canine
• Source: HEK293
• Tag: His
• Protein Length: 19-236 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 26.6 kDa. The protein migrates as 35-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Phe19-Arg236
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.