Recombinant C. elegans trxr-1 Protein, 667 residues

• Cat.No.: trxr-1-17C
• Product Overview: Recombinant C. elegans Thioredoxin Reductase 1 (Non-tagged, 667 residues)

Specification

• Species: C.elegans
• ProteinLength: 667 residues
• Description: Predicted to enable thioredoxin-disulfide reductase activity. Involved in ecdysis, collagen and cuticulin-based cuticle. Predicted to be located in cytosol. Predicted to be active in mitochondrion. Is expressed in several structures, including epithelial cell; intestine; nervous system; pharynx; and vulva. Human ortholog(s) of this gene implicated in amyotrophic lateral sclerosis. Orthologous to human TXNRD1 (thioredoxin reductase 1) and TXNRD3 (thioredoxin reductase 3).
• Purity: ≥ 95% as judged by gel analysis
• Unit definition: 1 U of enzyme reduces 1 μmol DTNB per min in 0.5 mL standard DTNB assay containing 2.5 mM DTNB and 0.3 mM NADPH in TE buffer (50 mM Tris-HCl, 2 mM EDTA, pH 7.5)
• Applications: DTNB assay; Insulin-coupled Thioredoxin assay; Inhibitor screening; etc.
• Notes: Centrifuge the tube briefly before opening the lid.
• Concentration: 1 mg/mL
• Storage Buffer: TE buffer with 50% glycerol
• Shipping: Shipped in cold pack by courier service. Store at -20 centigrade upon delivery.
• References: 1. Cheng Q, Arnér ES. (2017) Selenocysteine Insertion at a Predefined UAG Codon in a Release Factor 1 (RF1)-depleted Escherichia coli Host Strain Bypasses Species Barriers in Recombinant Selenoprotein Translation. J Biol Chem. 292:5476-5487.
  2. Arnér ES, Holmgren A. (2001) Measurement of thioredoxin and thioredoxin reductase. Curr Protoc Toxicol. Chapter 7:Unit 7.4.

Gene Information

• Gene Name: trxr-1 Thioredoxin reductase 1;Thioredoxin-disulfide reductase [ Caenorhabditis elegans ]
• Official Symbol: trxr-1
• Synonyms: trxr-1; Thioredoxin reductase 1;Thioredoxin-disulfide reductase
• Gene ID: 177466
• mRNA Refseq: NM_001307310
• Protein Refseq: NP_001294239
• UniProt ID: Q17745