Recombinant Burkholderia Mallei BOPE Protein (1-261 aa), His-SUMO-tagged

• Cat.No.: BOPE-1969B
• Product Overview: Recombinant Burkholderia Mallei (strain ATCC 23344) BOPE Protein (1-261 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Protein Description: Full Length.

Specification

• Species: Burkholderia Mallei
• Source: E.coli
• Tag: His&SUMO
• Protein Length: 1-261 aa
• Description: Activator for both CDC42 and RAC1 by directly interacting with these Rho GTPases and acting as a guanine nucleotide exchange factor (GEF). This activation results in actin cytoskeleton rearrangements and stimulates membrane ruffling, thus promoting bacterial entry into non-phagocytic cells.
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 44.7 kDa
• AA Sequence: MTYNPRIGGFTHVKQASFDVHVKRGEAQPRTSFAQQIKRIFSKIGETLGQLFRHRAPDSAPGRVRLQGVRYVGSYRPTGDAKQAIRHFVDEAVKQVAHTRTPEIRQDAEFGRQVYEATLCAIFSEAKDRFCMDPATRAGNVRPAFIEALGDAARATGLPGADKQGVFTPSGAGTNPLYTEIRLRADTLMGAELAARPEYRELQPYARQQAIDLVANALPAERSNTLVEFRQTVQTLEATYRRAAQDASRDEKGATNAADGA
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: bopE type III secretion target BopE [ Burkholderia mallei ATCC 23344 ]
• Official Symbol: BOPE
• Synonyms: bopE; Effector protein BopE
• Gene ID: 3087007
• Protein Refseq: YP_106114
• UniProt ID: Q62B14