Recombinant Brevibacillus sp Cas12b Protein, His-tagged

• Cat.No.: Cas12b-04B
• Product Overview: Recombinant Brevibacillus sp BrCas12b (1-1090) was expressed in E. coli cells with a C-terminal His tag.

Specification

• Description: CRISPR (clustered regularly interspaced short palindromic repeat) and CRISPR-associated (Cas) proteins constitute the adaptive immune system in bacteria. This system has been redesigned to create an exemplary genome editing tool for RNA-based therapeutics development. Cas12 and Cas13 have collateral nucleotide cleavage activities and are widely used in CRISPR-based diagnostic platforms. BrCas12b from Brevibacillus shows robust trans-cleavage activity at ideal LAMP (loop-mediated isothermal amplification) temperature. The thermal stability of BrCas12b enables one-pot target nucleic detection to minimize carryover contamination.
• Source: E. coli
• Species: Brevibacillus Sp
• Tag: His
• Protein length: 1-1090
• Stability: One year at -70 centigrade from dare of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.23 μg/μL
• Storage Buffer: 150mM imidazole, 50mM sodium phosphate, pH 7.5, 1mM DTT, 10% glycerol.

Gene Information

• Official Symbol: Cas12b
• Synonyms: c2c1; CRISPR-associated endonuclease C2c1; Cas12b
• Protein Refseq: WP_165214399