Recombinant Bovine PDGF-BB Protein, Ser82-Thr190

• Cat.No.: Pdgfb-23B
• Product Overview: Recombinant Bovine PDGF-BB Protein (Ser82-Thr190) without tag was expressed in P. pastoris.

Specification

• Species: Bovine
• Source: P.pastoris
• Protein Length: Ser82-Thr190
• Description: Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in an animal study by Lynch and co-workers in the late 1980s. It is a disulfide-linked dimer consisting of two peptides-chain A and chain B. PDGF has three subforms: PDGF-AA, PDGF-BB, and PDGF-AB. It is involved in many biological processes, including hyperplasia, embryonic neuron development, chemotaxis, and respiratory tubule epithelial cell development. The function of PDGF is mediated by two receptors (PDGFR-α and PDGFR-β).
• Bio-activity: ED50 < 20.00 ng/ml as determined by a dose-response proliferation assay using murine Balb/c 3T3 cells. Based on the ED50, the calculated specific activity is approximately > 0.5 × 105 IU/mg. It is recommended to experimentally determine the optimal concentration for each specific application by performing a dose response assay.
• Molecular Mass: Theoretical Molecular Weight: 12 kDa
  Apparent Molecular Weight: 10-18 kDa, on SDS-PAGE under reducing conditions.
• Endotoxin: < 0.1 EU/μg of protein by gel clotting method
• Purity: ≥ 95% as analyzed by SDS-PAGE
• Storage: Upon receiving, the lyophilized product remains stable for up to 6 months at lower than -70 centigrade. Upon reconstitution, the product is stable for up to 1 week at 4 centigrade or up to 3 months at -20 centigrade. Avoid repeated freeze-thaw cycles by making single-use aliquots before the solution is storage at -20 centigrade.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in 20 mM NaAc, pH 5.0.
• Reconstitution: Before opening, centrifuge the vial briefly to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH2O up to 100 μg/mL.

Gene Information

• Gene Name: PDGFB platelet derived growth factor subunit B [ Bos taurus (cattle) ]
• Official Symbol: PDGFB
• Synonyms: PDGFB; platelet derived growth factor subunit B; platelet-derived growth factor subunit B; platelet-derived growth factor B; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog); v-sis platelet-derived growth factor beta polypeptide (simian sarcoma viral oncogene homolog)
• Gene ID: 540106
• mRNA Refseq: NM_001017953
• Protein Refseq: NP_001017953
• UniProt ID: B1H0W5